Andy Murray withdrew from Wimbledon with a "heavy heart" after saying his right hip was still not ready for the demands of a Grand Slam.
Murray made a comeback last month at Queen's Club following almost a year out with the injury that required surgery in Australia in January.
During a news conference he sounded hopeful about his chances of being ready to face Frenchman Benoit Paire in the first round on Tuesday.
But he announced he was pulling out 24 hours later having consulted his team. It is the first time Murray has missed Wimbledon since 2007 when a wrist injury ruled him out.
"It is with a heavy heart that I'm announcing that I'll be withdrawing from Wimbledon this year," Murray, who ended a 77-year wait for a British men's champion at Wimbledon when he beat Novak Djokovic in the 2013 final, said on Twitter.
"I've made significant progress in practice and in matches over the last 10 days, but after lengthy discussions with my team we've decided that playing best-of-five-set matches might be a bit too soon in the recovery process.
"We did everything we could to try and be ready in time."
On a more positive note Murray said he would continue practicing on Monday and would now target a return in the U.S. hardcourt season in time for the U.S. Open.
"I will start practicing on the hard courts from tomorrow and continuing with my rehab and recovery and I'm looking forward to the U.S. hardcourt season," he said.
"Thanks for all the messages of support and I'm excited to finally be back playing after so long out."
Murray admitted that he needed to take a smarter approach to protecting his body.
While Murray lost his opening comeback match against Nick Kyrgios at Queen's Club, he beat Stan Wawrinka at Eastbourne last week before losing to new British number one Kyle Edmund in straight sets.
His place in the Wimbledon draw was taken by lucky loser Jason Jung of Taiwan.
